Tree Zone Trek is the 2nd secret level in the Mushroom Kingdom, and is created by AuraLancer. It's based on the Super Mario Land 2 Tree Zone levels. It's similar to a previous Tree Zone level included in earlier versions of the game, but it's much longer and encompasses more of the different settings of the Tree Zone, including swimming through the tree sap in the base of the tree, navigating in the air on the heads of the owls flying around, and working through a bee hive. A brief auto-scrolling section also appears early on.
Radonkel, the crow boss from the original Tree Zone, serves as the level boss.